Applications
Extraction and Analysis of Metronidazole in Facial Cleansers
20631
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Antiprotozoal Agents
Personal Care/Consumer Products
Personal Healthcare / Cosmetic
Product / Trade Good

Applications

Extraction and Analysis of Metronidazole in Facial Cleansers

Analytes

1
2
Compound Name
Metronidazole
CID
4173
Molecular Formula
C6H9N3O3
Molecular Weight
171
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 20631)
Column:Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4496-AN
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic Acid
B: MeOH
Gradient Profile:
Step No.Time(min)%A%B
10955
226040
336040
43.1955
55955
Flow Rate:300 µL/min
Temperature:25°C
Detection:Mass Spectrometer (MS) amu @ 650°C

Sample Preparation Details
Description:Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S029-TBJ
Pre Treatment Note:Evaporate to dryness at 50 C. Reconstitute in 200 uL of 10:90 MeOH:0.1% Formic Acid
SPE Condition 1:1 mL of MeOH
SPE Condition 2:1 mL of 0.1N HCl
SPE Load:Load Sample
SPE Wash 1:3 mL of 0.1N HCl
SPE Wash 2:3 mL of MeOH
SPE Wash 3:6 mL of Ethyl Acetate
SPE Dry:5 min under full vacuum
SPE Elution 1:1 mL of 5:95 NH4OH:MeOH (v:v)
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:200 µL 10:90 MeOH:0.1% Formic Acid

Order Items Used in This Application

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