Applications
Extraction and Analysis of Metronidazole in Facial Cleansers
20631
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Antiprotozoal Agents
Personal Care/Consumer Products
Personal Healthcare / Cosmetic
Product / Trade Good

Applications

Extraction and Analysis of Metronidazole in Facial Cleansers
Analytes
1
2
Compound Name
Metronidazole
Details
LC Conditions (App ID: 20631)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic Acid
B: MeOH
Gradient Profile:
Step No.Time(min)%A%B
10955
226040
336040
43.1955
55955
Flow Rate:
300 µL/min
Temperature:
25°C
Detection:
Mass Spectrometer (MS) amu @ 650°C
Sample Preparation Details
Description:
Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:
Pre Treatment Note:
Evaporate to dryness at 50 C. Reconstitute in 200 uL of 10:90 MeOH:0.1% Formic Acid
SPE Condition 1:
1 mL of MeOH
SPE Condition 2:
1 mL of 0.1N HCl
SPE Load:
Load Sample
SPE Wash 1:
3 mL of 0.1N HCl
SPE Wash 2:
3 mL of MeOH
SPE Wash 3:
6 mL of Ethyl Acetate
SPE Dry:
5 min under full vacuum
SPE Elution 1:
1 mL of 5:95 NH4OH:MeOH (v:v)
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL 10:90 MeOH:0.1% Formic Acid

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