Applications
Sulfonamides in Meat by LC/MS/MS
20036
Antiprotozoal Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Sulfonamides in Meat by LC/MS/MS
Analytes
1
2
3
4
5
6
7
8
Compound Name
Sulfadiazine
CID
5215
Molecular Formula
C10H10N4O2S
Molecular Weight
250
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 20036)
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic Acid in Water
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10982
22982
32.18515
46.55050
57.10100
680100
78.1982
810982
Flow Rate:
800 µL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) @ 300°C
Sample Preparation Details
SPE Condition 1:
4 mL Methanol
SPE Condition 2:
4 mL 0.1% Formic Acid in Water
SPE Load:
Load 4.00 mL Sample diluted in 0.1% Formic Acid/Methanol (95:5)
SPE Wash 1:
2 mL 0.1% Formic Acid in Water
SPE Dry:
2min at 10inches Hg
SPE Elution 1:
1 mL Methanol
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
250 µL 90:10 water:methanol, containing 0.1% formic acid
Similar Applications
search
No Data