Applications
Tetracyclines in Meat by LC/MS/MS
20032
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antimalarials
Pharmaceutical
Therapeutic / Clinical

Applications

Tetracyclines in Meat by LC/MS/MS

Analytes

1
2
3
4
5
Compound Name
Oxytetracycline
CID
5280972
Molecular Formula
C22H24N2O9
Molecular Weight
460
No. Hydrogen Bond Acceptors
11
No. Hydrogen Bond Donors
7

Conditions

LC Conditions (App ID: 20032)
Column:Gemini 5 µm C18 110 Å, LC Column 50 x 4.6 mm, Ea
Brand Name:Gemini
Part No:00B-4435-E0
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic Acid in Water
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10982
22982
32.18515
46.55050
57.10100
680100
78.1982
810982
Flow Rate:0.8 mL/min
Temperature:40°C
Detection:Tandem Mass Spec (MS-MS) @ 600°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S100-UBJ
SPE Condition 1:4 mL Methanol at 30 µL/min
SPE Condition 2:4 mL 0.1% Formic Acid at 30 µL/min
SPE Load:Load 4.00 mL Sample diluted in Filtrate
SPE Wash 1:2 mL 0.1% Formic Acid at 30 µL/min
SPE Wash 2:1
SPE Dry:Evaporate to dryness using an eppendorf concentrator set at 45C or blow dry with nitrogen.
SPE Elution 1:1 mL Methanol
SPE Evaporation:Evaporate to dryness

Order Items Used in This Application

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