Applications
Penicillins in Meat by LC/MS/MS
20028
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Bacterial Agents

Applications

Penicillins in Meat by LC/MS/MS

Analytes

1
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8
Compound Name
Amoxicillin
CID
33613
Molecular Formula
C16H19N3O5S
Molecular Weight
365
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4

Conditions

LC Conditions (App ID: 20028)
Column:Gemini 3 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Gemini
Part No:00F-4439-E0
Elution Type:Gradient
Mobile Phase:A: 0.1% Ammonia in water
B: 95% Acetronitrile, 5% water and 0.1% ammonia
Gradient Profile:
Step No.Time(min)%A%B
10982
22982
32.429010
465050
56.10100
66.60100
76.8982
88982
Flow Rate:0.8 mL/min
Temperature:40°C
Detection:Tandem Mass Spec (MS-MS) V @ 600°C

Sample Preparation Details
Description:Strata C18-E (55 µm, 70 Å), 200 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S001-FBJ
Pre Treatment Note:The equilibration time at conditon 0 was 4 minutes before each injection.
SPE Condition 1:4 mL Methanol at 30 µL/min
SPE Condition 2:4 mL Water at 30 µL/min
SPE Load:Load 2.00 mL Sample diluted in Filtrate
SPE Wash 1:4 mL 0.1% Formic Acid in water
SPE Elution 1:2 mL Acetonitrile at 30 µL/min
SPE Elution 2:2 mL Acetonitrile at 30 µL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:200 µL Water

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