Applications
Chloramphenicol in Seafood and dairy products by LC/MS/MS
20026
Anti-Bacterial Agents
Food & Beverage
Pharmaceutical
Therapeutic / Clinical

Applications

Chloramphenicol in Seafood and dairy products by LC/MS/MS
Analytes
1
2
3
Compound Name
Chloramphenicol
CID
5959
Molecular Formula
C11H12Cl2N2O5
Molecular Weight
323
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 20026)
Elution Type:
Gradient
Mobile Phase:
A: Water
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
1-2955
20955
32955
42.01595
54.5595
Flow Rate:
0.4 mL/min
Temperature:
20°C
Detection:
Tandem Mass Spec (MS-MS) @ 450°C
Sample Preparation Details
SPE Condition 1:
3 mL Methanol at 1 mL/min
SPE Condition 2:
3 mL Water at 1 mL/min
SPE Load:
Load 10.00 mL Sample
SPE Wash 1:
3 mL Water at 1 mL/min
SPE Dry:
5 minutes @ 10 in Hg Vaccum
SPE Elution 1:
3 mL Ethyl Acetate at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
500 µL 0.5 mL Water/Acetonitrile (80:20)