Applications
Chloramphenicol in Seafood and dairy products by LC/MS/MS
20026
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Bacterial Agents
Food & Beverage
Pharmaceutical
Therapeutic / Clinical

Applications

Chloramphenicol in Seafood and dairy products by LC/MS/MS
Analytes
1
2
3
Compound Name
Chloramphenicol
Details
LC Conditions (App ID: 20026)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: Water
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
1-2955
20955
32955
42.01595
54.5595
Flow Rate:
0.4 mL/min
Temperature:
20°C
Detection:
Tandem Mass Spec (MS-MS) @ 450°C
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
SPE Condition 1:
3 mL Methanol at 1 mL/min
SPE Condition 2:
3 mL Water at 1 mL/min
SPE Load:
Load 10.00 mL Sample
SPE Wash 1:
3 mL Water at 1 mL/min
SPE Dry:
5 minutes @ 10 in Hg Vaccum
SPE Elution 1:
3 mL Ethyl Acetate at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
500 µL 0.5 mL Water/Acetonitrile (80:20)

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