Applications
Aldosterone on Kinetex 2.6 um XB-C18 50x3 mm ID
20009
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Anti-Inflammatory Agents
Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Aldosterone on Kinetex 2.6 um XB-C18 50x3 mm ID
Analytes
1
2
Compound Name
Aldosterone
CID
5839
Molecular Formula
C21H28O5
Molecular Weight
360
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 20009)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: DI Water
B: Methanol/Acetonitrile (50:50)
Gradient Profile:
Step No.Time(min)%A%B
107030
241090
351090
45.17030
577030
Flow Rate:
500 µL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C
Sample Preparation Details
Description:
Strata-X-A 33 µm Polymeric Strong Anion, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
SPE Condition 1:
3 mL Methanol
SPE Condition 2:
3 mL 25 mM Ammonium bicarbonate
SPE Load:
Load 1.50 mL Sample diluted in 25 mM Ammonium bicarbonate (pH 8.9-9.0)
SPE Wash 1:
3 mL 25 mM Ammonium bicarbonate
SPE Wash 2:
3 mL 25% Methanol in water
SPE Dry:
5 min at high vacuum
SPE Elution 1:
1 mL Ethyl acetate/Isopropanol/Ammonium hydroxide (7:2:1)
SPE Elution 2:
1 mL Ethyl acetate/Isopropanol/Ammonium hydroxide (7:2:1)
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
100 µL Methanol/Water (30:70)

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Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 3 mm, Ea
Reversed Phase