Applications
Hydroxy Vitamin D Isomers on Kinetex 2.6um C18 30 x 3mm
20005
Vitamins
Clinical

Applications

Hydroxy Vitamin D Isomers on Kinetex 2.6um C18 30 x 3mm
Analytes
1
2
3
Compound Name
Calcifediol
CID
5283731
Molecular Formula
C27H44O2
Molecular Weight
400
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 20005)
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic Acid in DI H2O
B: 0.1% Formic Acid in ACN
Gradient Profile:
Step No.Time(min)%A%B
104555
20.32080
312080
41.1595
51.5595
61.514555
724555
Flow Rate:
1000 µL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C