Applications
Water-soluble Vitamins on Kinetex 2.6u, C18, 150x4.6 by HPLC
20000
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Vitamin B Complex
Food & Beverage
Therapeutic / Clinical
Product / Trade Good
Biochemical Compound / Nutrient
Product / Trade Good

Applications

Water-soluble Vitamins on Kinetex 2.6u, C18, 150x4.6 by HPLC

Analytes

1
2
3
4
5
6
7
8
9
10
11
12
13
Compound Name
Ascorbic Acid
CID
5785
Molecular Formula
C6H8O6
Molecular Weight
176
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4

Conditions

LC Conditions (App ID: 20000)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00F-4462-E0
Elution Type:Gradient
Mobile Phase:A: 3g Sodium 1-hexanesulfonate monohydrate/0.4mL Triethylamine/15mL Acetic acid in 1985mL H2O
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
10955
2125347
312.1298
415298
515.1955
622955
Flow Rate:0.5 mL/min
Temperature:25°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Order Items Used in This Application

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