Applications
THC and Metabolites from Whole Blood on Strata-X-Drug B and Kinetex C18 by LC-MS-MS
19947
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Psychotropic Drugs
Forensics/Toxicology
Chemical Substance
Therapeutic / Clinical

Applications

THC and Metabolites from Whole Blood on Strata-X-Drug B and Kinetex C18 by LC-MS-MS

Analytes

1
2
3
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6
Compound Name
Hexestrol Dibutyrate
CID
644022
Molecular Formula
C21H30O3
Molecular Weight
330
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 19947)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 1 mM ammonium formate with 0.1% FA
B: 0.1% FA/methanol:acetonitrile(1:1)
Gradient Profile:
Step No.Time(min)%A%B
105050
23595
34.5595
44.515050
565050
Flow Rate:0.4 mL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk
Part Number:8B-S128-UCH
Pre Treatment Note:Evaporate eluant to dryness under 50 °C nitrogen. Reconstitute in 500uL of initial mobile phase. Extraction was automated and performed using a Gilson GX-274 ASPEC system
SPE Load:Load Sample
SPE Wash 1:2mL of ACN:Water (15:85)
SPE Dry:5 mins at 7-15 psi Nitrogen
SPE Elution 1:2mL of Ethyl acetate:Isopropanol (85:15)
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:500 µL Initial mobile phase (A/B=50/50)

Order Items Used in This Application

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