Applications
Testosterone from Female Human Plasma by LC/MS/MS using Strata-X-A and Kinetex 1.7 µm C18
19762
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Androgens
Clinical

Applications

Testosterone from Female Human Plasma by LC/MS/MS using Strata-X-A and Kinetex 1.7 µm C18

Analytes

1
2
Compound Name
Testosterone
CID
6013
Molecular Formula
C19H28O2
Molecular Weight
288
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 19762)
Column:Kinetex 1.7 µm C18 100 Å, LC Column 30 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00A-4475-AN
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic Acid +1 mM Amm Formate in Water
B: 0.1% Formic Acid +1 mM Amm Formate in ACN
Gradient Profile:
Step No.Time(min)%A%B
109010
22.51090
33.51090
43.69010
Flow Rate:0.4 mL/min
Temperature:55°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata-X-A 33 µm Polymeric Strong Anion, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S123-TBJ
Pre Treatment Note:Following evaporation of elution solvent @ 50-55 C under gentle nitrogen stream; Add 50 uL 25% hydroxylamine solution and heat at 60-65 C for 5-10 min, then add 200 uL 5% aqueous formic acid and vortex the tubes Transfer the solution to autosampler vials and inject 25 uL on column
SPE Condition 1:2 mL Methanol
SPE Condition 2:2 mL Water
SPE Load:Load 1.25 mL Sample diluted in Water
SPE Wash 1:0.6 mL 50% Methanol in Water
SPE Dry:Dry for 5 min under high vacuum
SPE Elution 1:0.3 mL Methanol
SPE Elution 2:0.3 mL Methanol
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:250 µL 200 uL 5% aqueous formic acid

Order Items Used in This Application

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