Applications
Nicotine and its Metabolites in Urine By LC/MS/MS Using Strata-X-C and Gemini-NX C18
19734
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Nicotinic Agonists
Clinical

Applications

Nicotine and its Metabolites in Urine By LC/MS/MS Using Strata-X-C and Gemini-NX C18

Analytes

1
2
3
4
5
Compound Name
Nornicotine
CID
412
Molecular Formula
C9H12N2
Molecular Weight
148
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 19734)
Column:Gemini 3 µm NX-C18 110 Å, LC Column 50 x 2 mm, Ea
Brand Name:Gemini
Part No:00B-4453-B0
Elution Type:Gradient
Mobile Phase:A: 20mM Ammonium bicarbonate
B: 100% Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
232575
33.19010
459010
Flow Rate:500 µL/min
Temperature:25°C
Detection:Mass Spectrometer (MS) amu @ 550°C

Sample Preparation Details
Description:Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S029-UBJ
SPE Condition 1:2 mL 100% Methanol at 2 mL/min
SPE Condition 2:2 mL 20mM Ammonium acetate at 2 mL/min
SPE Load:Load 0.50 mL Sample diluted in 0.5 mL 20mM Ammonium acetate
SPE Wash 1:2 mL 20mM Ammonium acetate at 2 mL/min
SPE Wash 2:2 mL 30% Methanol at 2 mL/min
SPE Dry:5min at full vacuum
SPE Elution 1:4 mL 1.5% Ammonium hydroxide methonal solution 2x2 mL at 2 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:500 µL 10:90 Acentonitrile:20mM Ammonium bicarbonate

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