Applications
Barbiturates in urine using Strata-X-Drug N and Kinetex C18 2.6µm 100x2.1mm
19724
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Adjuvants, Anesthesia
Clinical
Forensics/Toxicology
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Barbiturates in urine using Strata-X-Drug N and Kinetex C18 2.6µm 100x2.1mm

Analytes

1
2
3
4
5
6
7

Analyte not found

Conditions

LC Conditions (App ID: 19724)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 100 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00D-4462-AN
Elution Type:Gradient
Mobile Phase:A: 5mM Ammonium acetate
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
209010
3105545
410.011090
5121090
612.019010
7169010
Flow Rate:400 µL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata-X-Drug N 100u Polymer RP, 100mg/6mL, 30/Pk
Part Number:8B-S129-ECH
Pre Treatment Note:Evaporate eluant to dryness under stream of N2 (50ºC). Reconstitute samples with 1mL of 10% acetonitrile
SPE Load:Load Sample
SPE Wash 1:2 mLs of 0.1N HCl
SPE Wash 2:2 mLs of Methanol/0.1N HCl (30:70)
SPE Dry:10 min under full vacuum
SPE Elution 1:2 mLs of Ethylacetate/IPA (85:15) (v:v)
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:1 mL 10% acetonitrile

Order Items Used in This Application

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