Applications
Benzodiazepines from urine using Strata-X-Drug N on a Kinetex C18 2.6µm, 50x2.1mm
19714
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antiemetics
Clinical
Forensics/Toxicology
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Benzodiazepines from urine using Strata-X-Drug N on a Kinetex C18 2.6µm, 50x2.1mm
Analytes
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Compound Name
Alpha-Hydroxyalprazolam
Details
LC Conditions (App ID: 19714)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid
B: 0.1% Formic acid in methanol
Gradient Profile:
Step No.Time(min)%A%B
106535
24595
34.016535
476535
Flow Rate:
300 µL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-Drug N 100u Polymer RP, 100mg/6mL, 30/Pk
Part Number:
Pre Treatment Note:
Evaporate eluant under a stream of N2 gas at 50ºC. Reconstitute with 1mL of 35% methanol
SPE Load:
Load Sample
SPE Wash 1:
2 mLs of Acetonitrile:Water (20:80) (v:v)
SPE Dry:
10 minutes @ full vacuum
SPE Elution 1:
2mLs of Ethyl acetate:Isopropanol (85:15) (v:v)
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
1 mL 35% methanol in water

Order Items Used in This Application

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