Applications
Benzodiazepines from urine using Strata-X-Drug N on a Kinetex C18 2.6µm, 50x2.1mm
19714
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antiemetics
Clinical
Forensics/Toxicology
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Benzodiazepines from urine using Strata-X-Drug N on a Kinetex C18 2.6µm, 50x2.1mm

Analytes

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Compound Name
Alpha-Hydroxyalprazolam
CID
162244
Molecular Formula
C17H13ClN4O
Molecular Weight
324
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 19714)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic acid
B: 0.1% Formic acid in methanol
Gradient Profile:
Step No.Time(min)%A%B
106535
24595
34.016535
476535
Flow Rate:300 µL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata-X-Drug N 100u Polymer RP, 100mg/6mL, 30/Pk
Part Number:8B-S129-ECH
Pre Treatment Note:Evaporate eluant under a stream of N2 gas at 50ºC. Reconstitute with 1mL of 35% methanol
SPE Load:Load Sample
SPE Wash 1:2 mLs of Acetonitrile:Water (20:80) (v:v)
SPE Dry:10 minutes @ full vacuum
SPE Elution 1:2mLs of Ethyl acetate:Isopropanol (85:15) (v:v)
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:1 mL 35% methanol in water

Order Items Used in This Application

Similar Applications
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 3 mm, Ea
Reversed Phase
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase
Kinetex 2.6 µm Phenyl-Hexyl 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase
Luna 3 µm PFP(2) 100 Å, LC Column 50 x 3 mm, Ea
Reversed Phase
Zebron ZB-5MSi, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
Standard
Zebron ZB-5MSi, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
Standard
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
Application Specific
Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
Application Specific
Zebron ZB-5MSi, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
Standard
Zebron ZB-5MS, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea
Standard