Applications
LC/MS/MS of Extended Opiates from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm
19705
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Analgesics, Opioid
Forensics/Toxicology
Clinical

Applications

LC/MS/MS of Extended Opiates from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm

Analytes

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
Compound Name
Oxymorphone
CID
5284604
Molecular Formula
C17H19NO4
Molecular Weight
301
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 19705)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 5mM Ammonium acetate
B: 5mM Ammonium acetate in methanol
Gradient Profile:
Step No.Time(min)%A%B
108515
25.50100
36.50100
46.518515
598515
Flow Rate:500 µL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk
Part Number:8B-S128-UCH
Pre Treatment Note:Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 15% methanol.
SPE Load:Load Sample
SPE Wash 1:2mLs of 100mM Sodium acetate (pH 5.0) buffer
SPE Wash 2:2mLs of Methanol
SPE Dry:10 minutes @ full vacuum
SPE Elution 1:2mLs of Ethyl acetate:Isopropanol:NH4OH (7:2:1)
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:1 mL 15% methanol in water

Order Items Used in This Application

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