Applications
LC/MS/MS of Extended Opiates from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm
19705
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Analgesics, Opioid
Forensics/Toxicology
Clinical

Applications

LC/MS/MS of Extended Opiates from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm
Analytes
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
Compound Name
Oxymorphone
CID
5284604
Molecular Formula
C17H19NO4
Molecular Weight
301
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 19705)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 5mM Ammonium acetate
B: 5mM Ammonium acetate in methanol
Gradient Profile:
Step No.Time(min)%A%B
108515
25.50100
36.50100
46.518515
598515
Flow Rate:
500 µL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk
Part Number:
Pre Treatment Note:
Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 15% methanol.
SPE Load:
Load Sample
SPE Wash 1:
2mLs of 100mM Sodium acetate (pH 5.0) buffer
SPE Wash 2:
2mLs of Methanol
SPE Dry:
10 minutes @ full vacuum
SPE Elution 1:
2mLs of Ethyl acetate:Isopropanol:NH4OH (7:2:1)
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
1 mL 15% methanol in water

Order Items Used in This Application

Similar Applications
search
No Data