Applications
LC/MS/MS Analysis of Methadone from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm
19703
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Analgesics, Opioid
Forensics/Toxicology

Applications

LC/MS/MS Analysis of Methadone from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm
Analytes
1
2
Compound Name
Methadone
CID
4095
Molecular Formula
C21H27NO
Molecular Weight
309
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
2
Details
LC Conditions (App ID: 19703)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid
B: 0.1% Formic acid in acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
106040
23.50100
33.516040
46.56040
Flow Rate:
400 µL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk
Part Number:
Pre Treatment Note:
Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 40% acetonitrile.
SPE Load:
Load Sample
SPE Wash 1:
2 mLs of 100mM sodium acetate buffer (pH 5)
SPE Wash 2:
2 mLs of Methanol
SPE Dry:
10 minutes @ full vacuum
SPE Elution 1:
2mLs of Ethyl acetate:Isopropanol:NH4OH (7:2:1)
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
1 mL 40% acetonitrile in water

Order Items Used in This Application

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