Applications
Diclofenac from Plasma on Kinetex 2.6um C18 75x4.6mm
19701
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Inflammatory Agents

Applications

Diclofenac from Plasma on Kinetex 2.6um C18 75x4.6mm

Analytes

1
2
Compound Name
Flurbiprofen
CID
3394
Molecular Formula
C15H13FO2
Molecular Weight
244
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 19701)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 75 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00C-4462-E0
Elution Type:Isocratic
Mobile Phase:A: 63:37 Water(adjusted to pH 3.3 with phosphoric acid): Methanol
Gradient Profile:
Step No.Time(min)%A
15100
Flow Rate:1.5 mL/min
Temperature:30°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:8B-S100-TAK
SPE Condition 1:1 mL Methanol
SPE Condition 2:2 mL Water
SPE Load:Load 1.60 mL Sample diluted in Methanol:Water
SPE Wash 1:1 mL 5% Methanol in Water
SPE Elution 1:1 mL Methanol
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:500 µL 37:63 Methanol:Water

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