Applications
LC/MS/MS Analysis of Digoxin and Digitoxin in plasma (0.5 ng/mL) using Strata-X and Kinetex C8
19692
Separation Mode: Reversed Phase
Reversed Phase
C8
HPLC
Anti-Arrhythmia Agents
Clinical

Applications

LC/MS/MS Analysis of Digoxin and Digitoxin in plasma (0.5 ng/mL) using Strata-X and Kinetex C8

Analytes

1
2
3
Compound Name
Digoxin
CID
30322
Molecular Formula
C41H64O14
Molecular Weight
780
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
14
No. Hydrogen Bond Donors
6

Conditions

LC Conditions (App ID: 19692)
Column:Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4497-AN
Elution Type:Gradient
Mobile Phase:A: 10mM Ammonium acetate
B: 10mM Ammonium acetate in methanol
Gradient Profile:
Step No.Time(min)%A%B
105050
22.50100
32.515050
455050
Flow Rate:400 µL/min
Temperature:30°C
Detection:Mass Spectrometer (MS) amu @ 350°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S100-TBJ
SPE Condition 1:2 mL Methanol at 2 mL/min
SPE Condition 2:2 mL 10mM Ammonium acetate at 2 mL/min
SPE Load:Load 3.00 mL Sample diluted in Water
SPE Wash 1:1 mL 50:50 10mM ammonium acetate/10mM ammonium acetate in methanol at 2 mL/min
SPE Dry:10min at full vacuum
SPE Elution 1:2 mL Methanol at 1 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:100 µL 50:50 Methanol/water

Order Items Used in This Application

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