Applications
Estrone, Estradiol, and Estriol from Water @ pH 2.0 Strata-X via Kinetex XB-C18 150x4.60mm
19680
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Clinical

Applications

Estrone, Estradiol, and Estriol from Water @ pH 2.0 Strata-X via Kinetex XB-C18 150x4.60mm

Analytes

1
2
3
4
Compound Name
Estriol
CID
5756
Molecular Formula
C18H24O3
Molecular Weight
288
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 19680)
Column:Kinetex 2.6 µm XB-C18 100 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00F-4496-E0
Elution Type:Gradient
Mobile Phase:A: 6.9mM Ammonium Acetate pH 4.0 with 25% Acetonitrile (v/v)
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
101000
2103565
Flow Rate:1.2 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk
Part Number:8B-S100-FCH
SPE Condition 1:5 mL Acetonitrile at 2 mL/min
SPE Condition 2:5 mL Methanol at 2 mL/min
SPE Condition 3:10 mL MilliQ Water at 2 mL/min
SPE Load:Load 100.00 mL Sample diluted in Sample
SPE Wash 1:Not Required
SPE Dry:1 Minute
SPE Elution 1:10 mL Acetonitrile at 2 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:1 mL Methanol

Order Items Used in This Application

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