Applications
Estrone, Estradiol, and Estriol from Water @ pH 2.0 Strata-X via Kinetex XB-C18 150x4.60mm
19680
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Clinical

Applications

Estrone, Estradiol, and Estriol from Water @ pH 2.0 Strata-X via Kinetex XB-C18 150x4.60mm
Analytes
1
2
3
4
Compound Name
Estriol
Details
LC Conditions (App ID: 19680)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 6.9mM Ammonium Acetate pH 4.0 with 25% Acetonitrile (v/v)
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
101000
2103565
Flow Rate:
1.2 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk
Part Number:
SPE Condition 1:
5 mL Acetonitrile at 2 mL/min
SPE Condition 2:
5 mL Methanol at 2 mL/min
SPE Condition 3:
10 mL MilliQ Water at 2 mL/min
SPE Load:
Load 100.00 mL Sample diluted in Sample
SPE Wash 1:
Not Required
SPE Dry:
1 Minute
SPE Elution 1:
10 mL Acetonitrile at 2 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
1 mL Methanol

Order Items Used in This Application

Similar Applications
search
Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea
Reversed Phase
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea
Reversed Phase
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase
Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 4.6 mm, Ea
Reversed Phase
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 4.6 mm, Ea
Reversed Phase
Kinetex 2.6 µm Phenyl-Hexyl 100 Å, LC Column 50 x 4.6 mm, Ea
Reversed Phase
Kinetex 1.7 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase
Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase
Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase