Applications
Humic Acids using Kinetex C8 50x2.1 mm on ABI LC-MS 4000
19662
Separation Mode: Reversed Phase
Reversed Phase
C8
HPLC
Environmental

Applications

Humic Acids using Kinetex C8 50x2.1 mm on ABI LC-MS 4000

Analytes

1

Analyte not found

Conditions

LC Conditions (App ID: 19662)
Column:Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4497-AN
Elution Type:Gradient
Mobile Phase:A: 5mM Ammoniium Acetate
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
108515
22595
36595
46.018515
Flow Rate:0.4 mL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata PAH, 1.5 g / 6 mL, Tubes, 30/Pk
Part Number:8B-S130-7CH
SPE Condition 1:10 mL Dichloromethane at 5 mL/min
SPE Condition 2:20 mL Methanol at 5 mL/min
SPE Condition 3:20 mL MilliQ Water at 5 mL/min
SPE Load:Load 100.00 mL Sample diluted in 75:25 MilliQ Water:Acetonitrile
SPE Wash 1:5 mL 50:50 Methanol:MilliQ Water at 2 mL/min
SPE Dry:15 seconds
SPE Elution 1:6 mL Dichloromethane at 2 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:100 µL Dichlromethane

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