Applications
Humic Acids using Kinetex C8 50x2.1 mm on ABI LC-MS 4000
19662
Separation Mode: Reversed Phase
Reversed Phase
C8
HPLC
Environmental

Applications

Humic Acids using Kinetex C8 50x2.1 mm on ABI LC-MS 4000
Analytes
1

Analyte not found

Details
LC Conditions (App ID: 19662)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 5mM Ammoniium Acetate
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
108515
22595
36595
46.018515
Flow Rate:
0.4 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata PAH, 1.5 g / 6 mL, Tubes, 30/Pk
Part Number:
SPE Condition 1:
10 mL Dichloromethane at 5 mL/min
SPE Condition 2:
20 mL Methanol at 5 mL/min
SPE Condition 3:
20 mL MilliQ Water at 5 mL/min
SPE Load:
Load 100.00 mL Sample diluted in 75:25 MilliQ Water:Acetonitrile
SPE Wash 1:
5 mL 50:50 Methanol:MilliQ Water at 2 mL/min
SPE Dry:
15 seconds
SPE Elution 1:
6 mL Dichloromethane at 2 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
100 µL Dichlromethane

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