Applications
Fluoroquinolones by LCMS using Kinetex XB-C18 2.6µm, 50 x 2.1 mm
19656
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Antitubercular Agents
Food & Beverage
Environmental / Health
Product / Trade Good
Chemical Compound
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Fluoroquinolones by LCMS using Kinetex XB-C18 2.6µm, 50 x 2.1 mm
Analytes
1
2
3
4
5
6
7
8
Compound Name
Ofloxacin
Details
LC Conditions (App ID: 19656)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
108515
251090
361090
461090
Flow Rate:
0.4 mL/min
Temperature:
40°C
Detection:
Mass Spectrometer (MS) amu @ 550°C
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk
Part Number:
SPE Condition 1:
3 mL Methanol at 2 mL/min
SPE Condition 2:
3 mL Water at 2 mL/min
SPE Load:
Load 10.00 mL Sample diluted in Phosphate buffer with 2% TCA
SPE Wash 1:
2 at 2
SPE Dry:
5min @ >10 in Hg
SPE Elution 1:
2 mL Methanol/ACN/Acetic acid 49/49/2 at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
500 µL 0.1% Formic acid

Order Items Used in This Application

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