Applications
Fluoroquinolones by LCMS using Kinetex C8 2.6µm, 50 x 2.1 mm
19655
Separation Mode: Reversed Phase
Reversed Phase
C8
HPLC
Antitubercular Agents
Food & Beverage
Environmental / Health
Product / Trade Good
Chemical Compound
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Fluoroquinolones by LCMS using Kinetex C8 2.6µm, 50 x 2.1 mm

Analytes

1
2
3
4
5
6
7
8
Compound Name
Ofloxacin
CID
4583
Molecular Formula
C18H20FN3O4
Molecular Weight
361
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
8
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 19655)
Column:Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4497-AN
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic acid
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
108515
251090
361090
461090
Flow Rate:0.4 mL/min
Temperature:40°C
Detection:Mass Spectrometer (MS) amu @ 550°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk
Part Number:8B-S100-FCH
SPE Condition 1:3 mL Methanol at 2 mL/min
SPE Condition 2:3 mL Water at 2 mL/min
SPE Load:Load 10.00 mL Sample diluted in Phosphate buffer with 2% TCA
SPE Wash 1:2 at 2
SPE Dry:5min @ >10 in Hg
SPE Elution 1:2 mL Methanol/ACN/Acetic acid 49/49/2 at 1 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:500 µL 0.1% Formic acid

Order Items Used in This Application

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Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
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