Applications
6-MAM by LC/MS/MS using Strata-X-Drug B and a Kinetex C18 2.6µm, 50x2.1mm
19599
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Forensics/Toxicology

Applications

6-MAM by LC/MS/MS using Strata-X-Drug B and a Kinetex C18 2.6µm, 50x2.1mm
Analytes
1
2
Compound Name
6-Acetylmorphine
Details
LC Conditions (App ID: 19599)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
213070
333070
433070
Flow Rate:
400 µL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk
Part Number:
Pre Treatment Note:
Evaporate eluant to dryness and reconstitute in 1mL of 10% methanol in formic acid (0.1%)
SPE Load:
Load 4.00 mL Sample diluted in 100mM Phosphate buffer
SPE Wash 1:
2mL of 100mM sodium acetate buffer pH 5.0
SPE Wash 2:
2mL of Methanol
SPE Dry:
10 min at 10 inches of Hg
SPE Elution 1:
2mL of Ethyl acetate: IPA:NH4OH (7:2:1)
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
1000 µL 10% methanol in formic acid (0.1%)

Order Items Used in This Application

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