Applications
Phencyclidine from urine using Strata-X-Drug B and Kinetex C18, 50 x 2.1 mm by LCMS
19595
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Hallucinogens

Applications

Phencyclidine from urine using Strata-X-Drug B and Kinetex C18, 50 x 2.1 mm by LCMS
Analytes
1
2

Analyte not found

Details
LC Conditions (App ID: 19595)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid
B: Methanol with 0.1% Formic Acid
Gradient Profile:
Step No.Time(min)%A%B
107525
21595
32595
42.17525
547525
Flow Rate:
400 µL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) @ 500°C
Sample Preparation Details
Description:
Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 6 mL, Tubes, 30/Pk
Part Number:
Pre Treatment Note:
Evaporate eluant to dryness and reconstitute in 1 mL of 20% methanol in water. Phencyclidine was spiked into the urine at the 25ng/mL cutoff.
SPE Condition 1:
2 at 2
SPE Condition 2:
2 at 2
SPE Load:
Load 4.00 mL Sample diluted in Pre-treated Sample
SPE Wash 1:
2mL of 100mM Sodium acetate buffer (pH 5.0)
SPE Wash 2:
2mL of Methanol
SPE Dry:
10min @ >10 in Hg
SPE Elution 1:
2mL of Ethyl Acetate: IPA:NH4OH (7:2:1)
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
1 mL 20% methanol in water

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