Applications
USP Hydrocortisone Acetate on Kinetex 2.6µm XB-C18 75x4.6mm
19517
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Anti-Inflammatory Agents

Applications

USP Hydrocortisone Acetate on Kinetex 2.6µm XB-C18 75x4.6mm

Analytes

1
Compound Name
Hydrocortisone
CID
5754
Molecular Formula
C21H30O5
Molecular Weight
362
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 19517)
Column:Kinetex 2.6 µm XB-C18 100 Å, LC Column 75 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00C-4496-E0
Elution Type:Gradient
Mobile Phase:A: 80:20 Water:Acetonitrile
B: 70:30 Acetonitrile:Water
Gradient Profile:
Step No.Time(min)%A%B
109010
259010
3251090
4301090
5359010
6409010
Flow Rate:1 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

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