Applications
Tetrahydroiso-alpha acids extracted from beer by Strata-X using Kinetex C18 2.6µm 100x4.6 mm
19486
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC

Applications

Tetrahydroiso-alpha acids extracted from beer by Strata-X using Kinetex C18 2.6µm 100x4.6 mm

Analytes

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Analyte not found

Conditions

LC Conditions (App ID: 19486)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00D-4462-E0
Elution Type:Isocratic
Mobile Phase:A: Methanol
B: Water
C: Phosphoric acid
Gradient Profile:
Step No.Time(min)%A%B%C
1075241
Flow Rate:1.4 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk
Part Number:8B-S100-FCH
SPE Condition 1:4 mL Acidified methanol (0.05% H3PO4) at 1 mL/min
SPE Condition 2:4 mL Water at 1 mL/min
SPE Load:Load 5.00 mL Sample diluted in Miller Genuine Draft
SPE Wash 1:4 mL 40% methanol in water at 1 mL/min
SPE Dry:5 min @ >10 inches of Hg
SPE Elution 1:2 mL Acidified methanol at 1 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:500 µL Mobile phase

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