Applications
Iso-alpha acids extracted from beer by Strata-X using Kinetex C18 2.6µm 100x4.6 mm
19485
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC

Applications

Iso-alpha acids extracted from beer by Strata-X using Kinetex C18 2.6µm 100x4.6 mm
Analytes
1
2
3

Analyte not found

Details
LC Conditions (App ID: 19485)
Column:
Brand Name:
Part No:
Elution Type:
Isocratic
Mobile Phase:
A: Methanol
B: Water
C: Phosphoric acid
Gradient Profile:
Step No.Time(min)%A%B%C
1075241
Flow Rate:
1.4 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk
Part Number:
SPE Condition 1:
4 mL Acidified methanol (0.05% H3PO4) at 1 mL/min
SPE Condition 2:
4 mL Water at 1 mL/min
SPE Load:
Load 5.00 mL Sample diluted in Red Stripe Beer
SPE Wash 1:
4 mL 40% methanol in water at 1 mL/min
SPE Dry:
5 min @ >10 inches of Hg
SPE Elution 1:
2 mL Acidified methanol at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
500 µL Mobile phase

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