Applications
0.1 ng/g Chloramphenicol in Shrimp using Strata-X and Kinetex C18 50 x 2.1 mm by LCMS
19453
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Bacterial Agents
Food & Beverage
Pharmaceutical
Therapeutic / Clinical

Applications

0.1 ng/g Chloramphenicol in Shrimp using Strata-X and Kinetex C18 50 x 2.1 mm by LCMS
Analytes
1
2
3
Compound Name
Chloramphenicol
Details
LC Conditions (App ID: 19453)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 5 mM Ammonium bicarbonate
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
22955
32.01595
44.5595
Flow Rate:
0.4 mL/min
Temperature:
22°C
System:
Agilent (HP) 1200
Detection:
Mass Spectrometer (MS) @ 450°C
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
SPE Condition 1:
2 mL Methanol at 2 mL/min
SPE Condition 2:
2 mL Water at 2 mL/min
SPE Load:
Load 10.00 mL Sample diluted in Water
SPE Wash 1:
2 mL Water at 2 mL/min
SPE Dry:
5min @ >10 in Hg
SPE Elution 1:
Ethyl Acetate
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
500 µL 20% ACN in water

Order Items Used in This Application

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