Applications
Chloramphenicol in Shrimp Using Strata-X and Kinetex C18, 50 x 2.1 mm by LCMS
19302
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Bacterial Agents
Food & Beverage

Applications

Chloramphenicol in Shrimp Using Strata-X and Kinetex C18, 50 x 2.1 mm by LCMS

Analytes

1
2
Compound Name
Chloramphenicol
CID
5959
Molecular Formula
C11H12Cl2N2O5
Molecular Weight
323
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 19302)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 5 mM Ammonium bicarbonate
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
22955
32.01595
44.5595
Flow Rate:0.4 mL/min
Temperature:22°C
System:Agilent (HP)
Detection:Mass Spectrometer (MS) @ 450°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S100-UBJ
SPE Condition 1:2 mL Methanol at 2 mL/min
SPE Condition 2:2 mL Water at 2 mL/min
SPE Load:Load 10.00 mL Sample diluted in Water
SPE Wash 1:2 mL Water at 2 mL/min
SPE Dry:5min @ >10 in Hg
SPE Elution 1:Ethyl Acetate
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:500 µL 20% ACN in water

Order Items Used in This Application

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