Applications
Extraction of Analgesics from Plasma Using Strata X-A on Kinetex 2.6u, C18, 50x2.1mm by LC-UV
19246
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Inflammatory Agents, Non-Steroidal
Clinical

Applications

Extraction of Analgesics from Plasma Using Strata X-A on Kinetex 2.6u, C18, 50x2.1mm by LC-UV
Analytes
1
2
3
4
Compound Name
Salicylic Acid
Details
LC Conditions (App ID: 19246)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 20 mM KH2PO4(pH 7.0)
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
109010
219010
31.013070
45.53070
Flow Rate:
0.4 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
Description:
Strata-X-A 33 µm Polymeric Strong Anion, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:
Pre Treatment Note:
Elution solvent was applied in 2 aliquots of 500uL followed by evaporation to dryness at 45 deg C under N2 and reconstitute with 500 uL initial mobile phase (20mm KH2PO4(PH 7.0)/ MeOH=90/10) spiked with 5ug/mL Fenoprofen (IS).
SPE Condition 1:
1 mL Methanol at 1 mL/min
SPE Condition 2:
1 mL Water at 1 mL/min
SPE Load:
Load 0.50 mL Sample diluted in Water
SPE Wash 1:
1 mL 50 mm Na acetate, PH 7.0 at 1 mL/min
SPE Wash 2:
1 mL Methanol at 1 mL/min
SPE Dry:
2 mins at 10" of Hg
SPE Elution 1:
1 mL 5% FA/MeOH at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
500 µL 20mm KH2PO4(PH 7.0)/ MeOH=90/10

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