Applications
Mycotoxins on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS in ESI Positive Mode (1)
18878
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Carcinogens
Food & Beverage

Applications

Mycotoxins on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS in ESI Positive Mode (1)
Analytes
1
2
3
4
5
Compound Name
Fumonisin B1
Details
LC Conditions (App ID: 18878)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid
B: 0.1% Formic acid in Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
107030
23595
34595
44.017030
567030
Flow Rate:
400 µL/min
Temperature:
25°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata Screen-C (55 µm, 70 Å), 100 mg / 3 mL, Tubes, 50/Pk
Part Number:
SPE Condition 1:
3 mL Methanol at 1 mL/min
SPE Condition 2:
3 mL Water at 1 mL/min
SPE Condition 3:
1 mL 100 mM phosphate buffer, pH 6 (adjust with phosphoric acid) at 1 mL/min
SPE Load:
Load 2.00 mL Sample diluted in 100 mm acetate buffer
SPE Wash 1:
2 mL Water at 1 mL/min
SPE Wash 2:
2 mL 20% acetonitrile/100 mm phosphate buffer, pH 6 at 1 mL/min
SPE Dry:
2-3 mins at 10" of Hg
SPE Elution 1:
2 mL Ethylacetate at 1 mL/min
SPE Elution 2:
2 mL Ethylacetate at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
1 mL Initial mob phase(A/B=65/35)

Order Items Used in This Application

Similar Applications
search
Gemini 5 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase