Applications
Pain panel extracted from human urine at cutoff - Kinetex C18 2.6µm 50x2.1mm LCMSMS
18850
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Analgesics, Opioid
Forensics/Toxicology
Clinical

Applications

Pain panel extracted from human urine at cutoff - Kinetex C18 2.6µm 50x2.1mm LCMSMS

Analytes

1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
Compound Name
Morphine
CID
5288826
Molecular Formula
C17H19NO3
Molecular Weight
285
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 18850)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile:
Step No.Time(min)%A%B
10955
21.34060
31.31595
41.51595
51.52955
62.2955
Flow Rate:1.2 mL/min
Temperature:25°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S029-TBJ
SPE Condition 1:1 mL methanol
SPE Condition 2:1 mL 100 mM ammonium acetate buffer (pH 5.95)
SPE Load:Load Sample
SPE Wash 1:2 x 1 mL 100 mM ammonium acetate buffer (pH 5.95)
SPE Wash 2:2 x 1 mL methanol
SPE Dry:5 mins under full vacuum
SPE Elution 1:2 x 0.5 mL 60:24:16 ethyl acetate / IPA / NH4OH
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:1 mL 1 mL Initial Mobile Phase

Order Items Used in This Application

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