Applications
Extraction of Benzodiazepines Using Strata Screen-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
18849
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Anxiety Agents
Forensics/Toxicology
Clinical

Applications

Extraction of Benzodiazepines Using Strata Screen-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Analytes

1
2
3
4
5
6
7
8
9
10
11
Compound Name
Alpha-Hydroxyalprazolam
CID
162244
Molecular Formula
C17H13ClN4O
Molecular Weight
324
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 18849)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 0.1%FA in H2O
B: 0.1%FA in Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
106535
226535
32.01595
42.3595
52.316535
64.16535
Flow Rate:450 µL/min
Temperature:25°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata Screen-C (55 µm, 70 Å), 100 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S016-EBJ
SPE Condition 1:3 mL Methanol at 1 mL/min
SPE Condition 2:3 mL Water at 1 mL/min
SPE Condition 3:1 mL 100 mM phosphate buffer, pH 6 (adjust with phosphoric acid) at 1 mL/min
SPE Load:Load 2.00 mL Sample diluted in 100 mm acetate buffer
SPE Wash 1:2 mL Water at 1 mL/min
SPE Wash 2:2 mL 20% acetonitrile/100 mm phosphate buffer, pH 6 at 1 mL/min
SPE Dry:2-3 mins at 10" of Hg
SPE Elution 1:2 mL Ethylacetate at 1 mL/min
SPE Elution 2:2 mL Ethylacetate at 1 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:1 mL Initial mob phase(A/B=65/35)

Order Items Used in This Application

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