Applications
Extraction of Steroids Using Strata-X on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
18848
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Androgens
Clinical

Applications

Extraction of Steroids Using Strata-X on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
Analytes
1
2
3
4
5
6
7
8
9
10
11
Compound Name
Triamcinolone
Details
LC Conditions (App ID: 18848)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1%FA in H2O
B: 0.1%FA in Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
108020
22.52080
32.51595
42.8595
52.818020
64.68020
Flow Rate:
450 µL/min
Temperature:
25°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:
Pre Treatment Note:
Blown down samples (after evaporation) were reconstituted in 100 uL of initial mobile phase (A/B=80/20).
SPE Condition 1:
1 mL Methanol at 2 mL/min
SPE Condition 2:
1 mL 0.1M Acetic acid at 2 mL/min
SPE Load:
Load 0.50 mL Sample diluted in 0.1M Acetic acid
SPE Wash 1:
1 mL 0.1M Acetic acid at 2 mL/min
SPE Wash 2:
1 mL 40% Methanol/water at 2 mL/min
SPE Dry:
2 mins at 10" of Hg pressure
SPE Elution 1:
0.5 mL Methanol at 1 mL/min
SPE Elution 2:
0.5 mL Methanol at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
100 µL Initial mobile phase (A/B=80/20)

Order Items Used in This Application

Similar Applications
search
Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea
Reversed Phase
Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea
Reversed Phase
Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea
Reversed Phase
Gemini 5 µm C18 110 Å, LC Column 250 x 3 mm, Ea
Reversed Phase
Onyx Monolithic C18, LC Column 100 x 4.6 mm, Ea
Reversed Phase
Onyx Monolithic C18, LC Column 100 x 4.6 mm, Ea
Reversed Phase