Applications
Extraction of Steroids Using Strata-X on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
18848
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Androgens
Clinical

Applications

Extraction of Steroids Using Strata-X on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Analytes

1
2
3
4
5
6
7
8
9
10
11
Compound Name
Triamcinolone
CID
31307
Molecular Formula
C21H27FO6
Molecular Weight
394
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
4

Conditions

LC Conditions (App ID: 18848)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 0.1%FA in H2O
B: 0.1%FA in Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
108020
22.52080
32.51595
42.8595
52.818020
64.68020
Flow Rate:450 µL/min
Temperature:25°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S100-TBJ
Pre Treatment Note:Blown down samples (after evaporation) were reconstituted in 100 uL of initial mobile phase (A/B=80/20).
SPE Condition 1:1 mL Methanol at 2 mL/min
SPE Condition 2:1 mL 0.1M Acetic acid at 2 mL/min
SPE Load:Load 0.50 mL Sample diluted in 0.1M Acetic acid
SPE Wash 1:1 mL 0.1M Acetic acid at 2 mL/min
SPE Wash 2:1 mL 40% Methanol/water at 2 mL/min
SPE Dry:2 mins at 10" of Hg pressure
SPE Elution 1:0.5 mL Methanol at 1 mL/min
SPE Elution 2:0.5 mL Methanol at 1 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:100 µL Initial mobile phase (A/B=80/20)

Order Items Used in This Application

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