Applications
Extraction of Amphetamines-NIDA5 Using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm by LCMS
18845
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Adrenergic Agents
Forensics/Toxicology
Clinical

Applications

Extraction of Amphetamines-NIDA5 Using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm by LCMS
Analytes
1
2
3
4
5
6
7
8
Compound Name
Norephedrine
Details
LC Conditions (App ID: 18845)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1%FA-5mM NH4Ac in H2O
B: 0.1%FA-5mM NH4Ac in Methanol
Gradient Profile:
Step No.Time(min)%A%B
109010
218020
337030
43.01595
53.3595
63.319010
75.19010
Flow Rate:
450 µL/min
Temperature:
25°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:
Pre Treatment Note:
Blown down samples (after evaporation) were reconstituted in 200 uL of initial mobile phase (A/B=90/10).
SPE Condition 1:
1 mL Methanol at 2 mL/min
SPE Condition 2:
1 mL Water at 2 mL/min
SPE Load:
Load 1.00 mL Sample diluted in 100 mM KH2PO4 (pH 6)
SPE Wash 1:
1 mL 0.1N HCl at 2 mL/min
SPE Wash 2:
1 mL Methanol at 2 mL/min
SPE Dry:
2 mins @ 10" of Hg pressure
SPE Elution 1:
0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
SPE Elution 2:
0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL Initial mobile phase (A/B=90/10)

Order Items Used in This Application

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