Applications
Extraction of Cocaine-NIDA5 Using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
18843
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Dopamine Uptake Inhibitors
Forensics/Toxicology

Applications

Extraction of Cocaine-NIDA5 Using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
Analytes
1
2
3
4

Analyte not found

Details
LC Conditions (App ID: 18843)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile:
Step No.Time(min)%A%B
109010
223070
32.01595
42.3595
52.319010
64.19010
Flow Rate:
450 µL/min
Temperature:
25°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:
Pre Treatment Note:
Blown down samples (after evaporation) were reconstituted in 200 uL of initial mobile phase (A/B=90/10).
SPE Condition 1:
1 mL Methanol at 2 mL/min
SPE Condition 2:
1 mL Water at 2 mL/min
SPE Load:
Load 1.00 mL Sample diluted in 100 mM KH2PO4 (pH 6)
SPE Wash 1:
1 mL 0.1N HCl at 2 mL/min
SPE Wash 2:
1 mL Methanol at 2 mL/min
SPE Dry:
2 mins @ 10" of Hg pressure
SPE Elution 1:
0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
SPE Elution 2:
0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL Initial mobile phase (A/B=90/10)

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