Applications
Extraction of Healthcare Opiates using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LC/MS
18716
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Analgesics, Opioid
Clinical

Applications

Extraction of Healthcare Opiates using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LC/MS
Analytes
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
Compound Name
Normorphine
CID
5359401
Molecular Formula
C16H17NO3
Molecular Weight
271
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 18716)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid and 5 mM ammonium acetate in H2O
B: 0.1% Formic acid and 5 mM ammonium acetate in 50-50ACN-Methanol
Gradient Profile:
Step No.Time(min)%A%B
10955
20.24955
33.362080
43.37595
53.68595
63.69955
75.55955
Flow Rate:
400 µL/min
Temperature:
25°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:
Pre Treatment Note:
Blown down samples (after evaporation) were reconstituted in 200 uL of initial mobile phase (A/B=95/5).
SPE Condition 1:
1 mL Methanol at 2 mL/min
SPE Condition 2:
1 mL Water at 2 mL/min
SPE Load:
Load 1.00 mL Sample diluted in 100 mM KH2PO4 (pH 6)
SPE Wash 1:
1 mL 0.1N HCl at 2 mL/min
SPE Wash 2:
1 mL Methanol at 2 mL/min
SPE Dry:
2 mins @ 10" of Hg pressure
SPE Elution 1:
0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
SPE Elution 2:
0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL Initial mobile phase (A/B=95/5)

Order Items Used in This Application

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