Applications
Extraction of Methadone using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
18649
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Analgesics, Opioid
Forensics/Toxicology

Applications

Extraction of Methadone using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
Analytes
1
2
Compound Name
Methadone
CID
4095
Molecular Formula
C21H27NO
Molecular Weight
309
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
2
Details
LC Conditions (App ID: 18649)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile:
Step No.Time(min)%A%B
10973
20.25973
32.75595
43595
53.01973
64.5973
Flow Rate:
500 µL/min
Temperature:
25°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:
SPE Condition 1:
1 mL Methanol at 1 mL/min
SPE Condition 2:
1 mL Water at 1 mL/min
SPE Load:
Load 1.00 mL Sample diluted in 1
SPE Wash 1:
1 mL 0.1N HCl at 2 mL/min
SPE Wash 2:
1 mL Methanol at 2 mL/min
SPE Dry:
2-3 mins @ 10" of Hg
SPE Elution 1:
0.5 mL 5% ammonium hydroxide/methanol at 1 mL/min
SPE Elution 2:
0.5 mL 5% ammonium hydroxide/methanol at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL Initial LC mobile phase 97/3

Order Items Used in This Application

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