Applications
Extraction of Sulfa drugs using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
18643
Antiprotozoal Agents

Applications

Extraction of Sulfa drugs using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
Analytes
1
2
3
4
5
Compound Name
Sulfadiazine
CID
5215
Molecular Formula
C10H10N4O2S
Molecular Weight
250
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 18643)
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile:
Step No.Time(min)%A%B
10955
20.24955
33.02595
43.3595
53.31955
64.97955
Flow Rate:
450 µL/min
Temperature:
25°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
SPE Condition 1:
1 mL Methanol at 2 mL/min
SPE Condition 2:
1 mL Water at 2 mL/min
SPE Load:
Load 0.50 mL Sample diluted in 0.5
SPE Wash 1:
1 mL 0.1M acetic acid at 2 mL/min
SPE Wash 2:
1 mL 25% methanol/0.1M acetic acid at 2 mL/min
SPE Wash 3:
1 at 2
SPE Dry:
2 min at 10"Hg
SPE Elution 1:
0.5 mL 5% ammonium hydroxide/methanol at 1 mL/min
SPE Elution 2:
0.5 mL 5% ammonium hydroxide/methanol at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL Initial LC mobile phase (95/5)
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