Applications
Extraction of Tetracyclines using strata X-CW on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
18640
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antiprotozoal Agents

Applications

Extraction of Tetracyclines using strata X-CW on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Analytes

1
2
3
Compound Name
Oxytetracycline
CID
5280972
Molecular Formula
C22H24N2O9
Molecular Weight
460
No. Hydrogen Bond Acceptors
11
No. Hydrogen Bond Donors
7

Conditions

LC Conditions (App ID: 18640)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile:
Step No.Time(min)%A%B
10973
20.24973
33.02595
43.3595
53.31973
64.97973
Flow Rate:450 µL/min
Temperature:25°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S035-TBJ
SPE Condition 1:1 mL Methanol at 2 mL/min
SPE Condition 2:1 mL Ammonium acetate pH 5.5 at 2 mL/min
SPE Load:Load 0.50 mL Sample diluted in 1 mL
SPE Wash 1:1 mL Ammonium acetate pH 5.5 at 2 mL/min
SPE Wash 2:1 mL Methanol at 1 mL/min
SPE Dry:Dry for 2 mins at 10" of Hg
SPE Elution 1:0.500 mL 2% FA/methanol at 1 mL/min
SPE Elution 2:0.5 mL 2% FA/methanol at 1 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:200 µL Initial LC mobile phase (97/3)

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