Applications
Extraction of Tetracyclines using strata X-CW on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
18640
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antiprotozoal Agents

Applications

Extraction of Tetracyclines using strata X-CW on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS
Analytes
1
2
3
Compound Name
Oxytetracycline
CID
5280972
Molecular Formula
C22H24N2O9
Molecular Weight
460
No. Hydrogen Bond Acceptors
11
No. Hydrogen Bond Donors
7
Details
LC Conditions (App ID: 18640)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile:
Step No.Time(min)%A%B
10973
20.24973
33.02595
43.3595
53.31973
64.97973
Flow Rate:
450 µL/min
Temperature:
25°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:
SPE Condition 1:
1 mL Methanol at 2 mL/min
SPE Condition 2:
1 mL Ammonium acetate pH 5.5 at 2 mL/min
SPE Load:
Load 0.50 mL Sample diluted in 1 mL
SPE Wash 1:
1 mL Ammonium acetate pH 5.5 at 2 mL/min
SPE Wash 2:
1 mL Methanol at 1 mL/min
SPE Dry:
Dry for 2 mins at 10" of Hg
SPE Elution 1:
0.500 mL 2% FA/methanol at 1 mL/min
SPE Elution 2:
0.5 mL 2% FA/methanol at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL Initial LC mobile phase (97/3)

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