Applications
Caffeine in Ground Coffee Beans on Kinetex 2.6µm C18 100 x 4.6mm ID
18532
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Phosphodiesterase Inhibitors

Applications

Caffeine in Ground Coffee Beans on Kinetex 2.6µm C18 100 x 4.6mm ID

Analytes

1
Compound Name
Caffeine
CID
2519
Molecular Formula
C8H10N4O2
Molecular Weight
194
No. Hydrogen Bond Acceptors
3

Conditions

LC Conditions (App ID: 18532)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00D-4462-E0
Elution Type:Gradient
Mobile Phase:A: 0.1% phosphoric acid in water
B: 0.1% phosphoric acid in acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
20.67955
32.676040
44.336040
55595
65.01955
77955
Flow Rate:1.8 mL/min
Temperature:30°C
Detection:UV-Vis Abs.-Diode Array (PDA) nm @ 22°C

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