Applications
Extraction of Sulfonamides from Honey by LC/MS/MS Using strata X-C and Gemini 5u, C18, 150x3.0
18458
Antiprotozoal Agents
Pesticide / Insecticide
Environmental / Health

Applications

Extraction of Sulfonamides from Honey by LC/MS/MS Using strata X-C and Gemini 5u, C18, 150x3.0
Analytes
1
2
3
4
5
Compound Name
Sulfanilamide
CID
5333
Molecular Formula
C6H8N2O2S
Molecular Weight
172
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 18458)
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid/water
B: 0.1% formic acid/ACN
Gradient Profile:
Step No.Time(min)%A%B
109010
283070
Flow Rate:
0.6 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Pre Treatment Note:
Addition of IS followed by evaporation before reconstitution: Add IS (Sulfaquinoxaline) and evaporate the sample under slow stream of nitrogen @ 40 deg C and reconstitute with 100 uL of mobile phase
SPE Condition 1:
2 mL Methanol at 1 mL/min
SPE Condition 2:
2 mL Water at 1 mL/min
SPE Load:
Load 1.00 mL Sample diluted in 1mL of 2M HCl and 3mL 0.3M Citric acid
SPE Wash 1:
4 mL Water (2 aliquots of 2 mL) at 1 mL/min
SPE Wash 2:
4 mL MeOH/ACN (50/50) (2 aliquots of 2 mL) at 1 mL/min
SPE Dry:
2 mins at 10" of Hg pressure
SPE Elution 1:
2 mL 2% ammonium hydroxide/MeOH at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0 µL Mobile Phase
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