Applications
Extraction of Sulfa Drugs from Porcine Plasma using Strata X-C
18293
Separation Mode: Reversed Phase
Reversed Phase
Max-RP
HPLC
Antiprotozoal Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Extraction of Sulfa Drugs from Porcine Plasma using Strata X-C

Analytes

1
2
3
4
Compound Name
Sulfanilamide
CID
5333
Molecular Formula
C6H8N2O2S
Molecular Weight
172
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 18293)
Column:Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Synergi
Part No:00F-4337-E0
Elution Type:Gradient
Mobile Phase:A: 20 mm KH2PO4 (pH2.5)
B: ACN
Gradient Profile:
Step No.Time(min)%A%B
10955
2152080
Flow Rate:1 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Sample Preparation Details
SPE Condition 1:1 mL Methanol at 1 mL/min
SPE Condition 2:1 mL Water at 1 mL/min
SPE Load:Load 1.50 mL Sample diluted in Water
SPE Wash 1:1 mL HCl (0.1N) at 1 mL/min
SPE Wash 2:1 mL Methanol at 1 mL/min
SPE Dry:1 min at 10" of Hg
SPE Elution 1:1 mL 5% Ammonium Hydroxide in Methanol at 1 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:200 µL KH2PO4 (20mM) pH 2.5

Order Items Used in This Application

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