Applications
Steroids in a Blank Urine Sample on Gemini 5µm C18 150 x 3mm ID
17972
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Inflammatory Agents
Clinical
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

Steroids in a Blank Urine Sample on Gemini 5µm C18 150 x 3mm ID

Analytes

1
2
3
4
5
6
7
8
9
10
Compound Name
Triamcinolone
CID
31307
Molecular Formula
C21H27FO6
Molecular Weight
394
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
4

Conditions

LC Conditions (App ID: 17972)
Column:Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea
Brand Name:Gemini
Part No:00F-4435-Y0
Elution Type:Gradient
Mobile Phase:A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in 95% Acetonitrile/Water
Gradient Profile:
Step No.Time(min)%A%B
106040
241000
3152575
415.14060
5204060
Flow Rate:0.6 mL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 450°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:8B-S100-TAK
SPE Condition 1:1 mL Methanol
SPE Condition 2:1 mL Water
SPE Load:Load 1.00 mL Sample diluted in Human serum or urine
SPE Wash 1:1 mL Water
SPE Wash 2:1 mL 40% Methanol in Water
SPE Elution 1:1 mL Methanol
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:100 µL 100 uL acetonitrile/water 40:60

Order Items Used in This Application

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