Applications
Polar Probes on Synergi Max
17943
Separation Mode: Reversed Phase
Reversed Phase
Max-RP
HPLC
Analgesics, Opioid
Chromatography
Chromatographic Property

Applications

Polar Probes on Synergi Max

Analytes

1
2
3
4
5
Compound Name
Guanine
CID
764
Molecular Formula
C5H5N5O
Molecular Weight
151
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 17943)
Column:Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Synergi
Part No:00F-4337-E0
Elution Type:Gradient
Mobile Phase:A: Phosphate Buffer, pH 2.5
B: Acetonitrile
C: 0
D: 0
Gradient Profile:
Step No.Time(min)%A%B
101000
251000
35.01955
4107030
510.51000
6141000
Flow Rate:1 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Diode Array (PDA) nm @ 22°C

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