Applications
THC and Metabolite in Plasma by LC/MS/MS on Gemini C18 5µm 50 x 3.0
17901
Psychotropic Drugs
Forensics/Toxicology
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

THC and Metabolite in Plasma by LC/MS/MS on Gemini C18 5µm 50 x 3.0
Analytes
1
2
3
Compound Name
Thc-11-Oic Acid
CID
107885
Molecular Formula
C21H28O4
Molecular Weight
344
No. Chiral Centers:
2
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 17901)
Elution Type:
Gradient
Mobile Phase:
A: 10mM NH4HCO3 (pH 10)
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10.18515
23595
33.5595
43.68515
54.18515
Flow Rate:
0.8 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 450°C
Sample Preparation Details
SPE Condition 1:
1 mL Methanol
SPE Condition 2:
1 mL DI Water
SPE Load:
Load 2.00 mL Sample diluted in 1mL Plasma diluted 1:1 with Water
SPE Wash 1:
1 mL 0.1N HAC
SPE Wash 2:
1 mL 30% ACN in 0.1N HAC
SPE Wash 3:
0.2
SPE Elution 1:
1 mL 2% HAC in CH3CN by gravity
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
100 µL 15:85/ACN:10mM NH4HCO3, pH=10.0
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