Applications
Amphetamines in Urine by LC/MS/MS on Gemini 5µm C18 150 x 3.0
17900
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Serotonin Agents
Clinical
Forensics/Toxicology
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

Amphetamines in Urine by LC/MS/MS on Gemini 5µm C18 150 x 3.0

Analytes

1
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7

Analyte not found

Conditions

LC Conditions (App ID: 17900)
Column:Gemini 5 µm C18 110 Å, LC Column 150 x 3 mm, Ea
Brand Name:Gemini
Part No:00F-4435-Y0
Elution Type:Isocratic
Mobile Phase:A: 10mM Ammonium formate w/0.1% formic acid
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
Flow Rate:0.6 mL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 450°C

Sample Preparation Details
Description:Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:8B-S029-TAK
Pre Treatment Note:to prevent the loss of amphetamine and methamphetamine during solvent evaporation, add two drops of conc. HCl before the evaporation step
SPE Condition 1:1 mL Methanol
SPE Condition 2:1 mL Water
SPE Load:Load Sample
SPE Wash 1:1 mL Water
SPE Wash 2:1 mL 0.1M Acetic Acid
SPE Wash 3:1 mL Methanol
SPE Elution 1:1 mL 2% of ammonium hydroxide (28-30%) in methanol
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:200 µL Mobile Phase

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