Applications
Polar bases (beta blockers) at high pH (ammonium bicarbonate pH 10.5) on Gemini-NX 5µ
16641
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Antihypertensive Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Polar bases (beta blockers) at high pH (ammonium bicarbonate pH 10.5) on Gemini-NX 5µ

Analytes

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Compound Name
Bisoprolol
CID
2405
Molecular Formula
C18H31NO4
Molecular Weight
325
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 16641)
Column:Gemini 5 µm NX-C18 110 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Gemini
Part No:00F-4454-E0
Elution Type:Gradient
Mobile Phase:A: 10mM Ammonium Bicarbonate pH 10.5
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
108515
2157030
3205050
4255050
Flow Rate:1.5 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

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