Applications
Nicotine in Ammonium Bicarbonate pH 10.5 on Gemini-NX 5µ
16627
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Nicotinic Agonists
Clinical
Pharmaceutical
Therapeutic / Clinical

Applications

Nicotine in Ammonium Bicarbonate pH 10.5 on Gemini-NX 5µ

Analytes

1
Compound Name
Nicotine
CID
89594
Molecular Formula
C10H14N2
Molecular Weight
162
No. Hydrogen Bond Acceptors
2

Conditions

LC Conditions (App ID: 16627)
Column:Gemini 5 µm NX-C18 110 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Gemini
Part No:00F-4454-E0
Elution Type:Gradient
Mobile Phase:A: 10 mM Ammonium Bicarbonate pH 10.5
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
210595
312595
Flow Rate:1.5 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

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