Applications
Simultaneous Desalting and Selective Elution of Basic, Neutral and Acidic Peptides
16087
Separation Mode: Reversed Phase
Reversed Phase
Max-RP
HPLC
Neurotransmitter Agents
Peptide
Biochemical Compound / Nutrient

Applications

Simultaneous Desalting and Selective Elution of Basic, Neutral and Acidic Peptides
Analytes
1
2
3
4

Analyte not found

Details
LC Conditions (App ID: 16087)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% TFA in Water
B: 0.1% TFA in Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
2123565
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:
SPE Condition 1:
1 mL Acetonitrile
SPE Condition 2:
Water
SPE Load:
Load 840.00 µL Sample diluted in water with salt
SPE Wash 1:
2 mL Water
SPE Elution 1:
0.25 mL 40:60 Acetonitrile/Water. for acidic peptides
SPE Elution 2:
0.25 mL 70:30 Acetonitrile/Water. for neutral peptides
SPE Elution 3:
0.25 mL 70:30 Acetonitrile/1.0% Formic Acid in Water. for basic peptides
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
20 µL initial mobile phase

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