Applications
Simultaneous Desalting and Selective Elution of Basic, Neutral and Acidic Peptides
16087
Separation Mode: Reversed Phase
Reversed Phase
Max-RP
HPLC
Neurotransmitter Agents
Peptide
Biochemical Compound / Nutrient

Applications

Simultaneous Desalting and Selective Elution of Basic, Neutral and Acidic Peptides

Analytes

1
2
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Analyte not found

Conditions

LC Conditions (App ID: 16087)
Column:Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Synergi
Part No:00F-4337-E0
Elution Type:Gradient
Mobile Phase:A: 0.1% TFA in Water
B: 0.1% TFA in Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
2123565
Flow Rate:1 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:8B-S100-TAK
SPE Condition 1:1 mL Acetonitrile
SPE Condition 2:Water
SPE Load:Load 840.00 µL Sample diluted in water with salt
SPE Wash 1:2 mL Water
SPE Elution 1:0.25 mL 40:60 Acetonitrile/Water. for acidic peptides
SPE Elution 2:0.25 mL 70:30 Acetonitrile/Water. for neutral peptides
SPE Elution 3:0.25 mL 70:30 Acetonitrile/1.0% Formic Acid in Water. for basic peptides
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:20 µL initial mobile phase

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