Applications
Simultaneous Desalting and Selective Elution of Basic, Acidic, and Neutral Peptides
14887
Vasodilator Agents
Peptide
Biochemical Compound / Nutrient

Applications

Simultaneous Desalting and Selective Elution of Basic, Acidic, and Neutral Peptides
Analytes
1
2
3
4
5

Analyte not found

Details
LC Conditions (App ID: 14887)
Elution Type:
Gradient
Mobile Phase:
A: 0.1% TFA in Water
B: 0.1% TFA in Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
2123565
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
SPE Condition 1:
1.0mL Acetonitrile
SPE Condition 2:
1.0mL Water
SPE Load:
Load 0.10 mL Sample diluted in Water
SPE Wash 1:
1.0 mL Water
SPE Elution 1:
0.25mL Acetonitrile:Water
SPE Elution 2:
0.25mL Acetonitrile:Water
SPE Elution 3:
0.25mL Acetonitrile:1% Formic Acid
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0.60 mL Mobile phase
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